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Enquiries & Booking

For more information regarding these systems please contact Ms. Jill McVee

bioImaging pricing
(password required)

bioImaging online Booking
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School of Biology bioImaging Facility

The Bio-Imaging facility is located in room A17 of the Bute Building. At the moment it contains two Leica Confocal microscope systems and in the future will also contain a Zeiss Axioplan 2 microscope which is currently available for use in C35.

Both Confocal systems are equipped with a Leica DM IRE2 inverted microscope with epifluoresence, bright field illumination, phase contrast and DIC.

Leica TCS SL

This system is the smaller of the two systems and incorporates three laser lines in the visible range. Ar. for excitation at 488-514 used with fluorescent dyes such as FITC, GFP ,YFP and  Alexa 488,HeNe 543-594 for TRITC and Cy3 and HeNe 633 for Texas Red, Cy5 and Alexa 633.

Leica Multi-Photon

This system has all the above laser lines plus a Mai Tai laser in the infra-red range which facilitates imaging of thicker specimens and is useful for live cell imaging as it has reduced photo toxicity. This system also has a 405 Diode laser in the Ultra-violet range which is useful for fluorescent dyes such as DAPI or Hoechst.

Zeiss Axioplan 2

This microscope can be used for digital colour/black and white imaging with a conventional microscope, as it is equipped with epifluoresence with filters for Blue, Green and Red fluorescence and Nomarski optics interfaced with Improvision software.



Gallery


Confocal 3D image Using DAF-2

P1 Trigeminal Neurone. Courtesy of Dr. G. Middleton.

Visualisation of Leptin Receptors

In P5 Trigeminal Neurones using FITC. Courtesy of Dr. G. Middleton

Mammalian cells 1/2

Expressing ABAD-EGFP fusion protein. Indicating the differing morphology of mitochondria in different cell types. Courtesy of Dr. F. Gunn-Moore

Mammalian cells 2/2

Expressing ABAD-EGFP fusion protein. Indicating the differing morphology of mitochondria in different cell types. Courtesy of Dr. F. Gunn-Moore

Confocal laser scanning microscope image

Of Chinese Hamster Ovary incubated with 100 nm gold particles. Courtesy of Dr. D. J. Stevenson

Some Publications including Confocal Images

Gunn-Moore F.J., Hill M., Davey F, Herron L.R., Tait S., Sherman D. & Brophy P.J.
A functional FERM-domain binding motif in neurofascin. Molecular & Cellular Neuroscience, 33, 441-6

Davey F, Hill M., Falk J, Sans N & Gunn-Moore FJ.
SAP102 is a novel binding partner to the cytoplasmic terminus of NrCAM.
(2005) Journal of Neurochemistry, 94, 1243-1253.

Gunn-Moore F., Welsh G., Herron L., Brannigan F., Venkateswarlu K., Brandwein-Gensler M., Madan R., Gillespie S., Tavar ., Brophy P., Prystowsky M., & Guild S.
A novel 4.1 ezrin radixin moesin (FERM) containing protein, Willin.
(2005) FEBS Letters, 579(22), 5089-94.

Doherty GH.
Developmental switch in the effects of TNFalpha on ventral midbrain dopaminergic neurons.
Neurosci Res. 2007 Feb;57(2):296-305. Epub 2006 Dec 5.

Williams HM, Lippock H, Doherty GH.
Nitric oxide and peroxynitrite signalling triggers homocysteine-mediated apoptosis in trigeminal sensory neurons in vitro.
Neurosci. Res. 2008. doi:10.1016/j.neures.2007.12.007 (in press - corrected proofs)

Alpert, M.H., Zhang, H., Molinari, M., Heitler, W.J., Sillar, K.T.
Nitric oxide modulation of the electrically excitable skin of Xenopus laevis frog tadpoles
* J. exp. Biol. 210, 3910-3918

Ramanathan,S., Combes, D., Molinari, M.,  Simmers, A.J., Sillar, K.T. (2006)
Developmental and regional expression of NADPH-diaphorase nitric oxide synthase in spinal cord neurons correlates with the emergence of limb motor networks in metamorphosing Xenopus laevis.
Eur. J. Neurosci. 1­17.