Dr Rona Ramsay

Reader

BMS 358 (Purdie)
tel. 01334 463411
fax. 01334 462595
rrr@st-andrews.ac.uk

Molecular Enzymology

Enzymology involves the structural and functional characterisation of proteins either in the cell or in purified form. Techniques of kinetic analysis, spectroscopy, structural determination, inhibitor studies, and molecular biology are combined to investigate how enzymes work.
We study particularly membrane bound mitochondrial enzymes. For example, mitochondrial monoamine oxidases A and B (MAO A and MAO B) are ubiquitous homodimeric FAD-containin oxidases that catalyse the oxidation of biogenic amines. Both enzymes play a vital role in the regulation of neurotransmitter levels in brain below) complement mechanistic studies of purified MAO A and its mutants to characterise the active site and the catalytic mechanism of the enzyme to aid in drug design.

Carnitine System

At the cellular level, investigating a family of homologous enzymes such as the carnitine
acyltransferases permits investigation of the molecular basis of regulation. Different
members of this family mediate fatty acid trafficking and influence pathways such as
ketogenesis, lipid synthesis and membrane repair (see scheme below). Current projects
include characterisation of the active sites of the acyltransferases by kinetic and mutagenic
techniques concurrent with crystallisation trials and, at a cellular level, a study of fluorescent
fusion proteins to investigate cellular trafficking.

External appointments

Honorary Fellow, Hannah Research Institute, Ayr, Scotland

Visiting Professor, Graduate Programme, University of Catania (2002-2006)

Basic Research Grant Panel, Science and Innovation, Enterprise Ireland (2002-4).

Presenter and Co-ordinator for Athena Women in Science events (from 2000).

Participant, Contemporary Poetry, Contemporary Science, Wellcome Trust

 

Publications

Bautista-Aguilera, ÓM, Hagenow, S, Palomino-Antolin, A, Farré-Alins, V, Ismaili, L, Joffrin, P-L, Jimeno, ML, Soukup, O, Janočková, J, Kalinowsky, L, Proschak, E, Iriepa, I, Moraleda, I, Schwed, JS, Romero Martínez, A, López-Muñoz, F, Chioua, M, Egea, J, Ramsay, RR, Marco-Contelles, J & Stark, H 2017, 'Multitarget-directed ligands combining cholinesterase and monoamine oxidase inhibition with histamine H3R antagonism for neurodegenerative diseases' Angewandte Chemie International Edition, vol 56, no. 41, 201706072R2, pp. 12765–12769 . DOI: 10.1002/anie.201706072
Ramsay, RR & Tipton, KF 2017, 'Assessment of enzyme inhibition: a review with examples from the development of monoamine oxidase and cholinesterase inhibitory drugs' Molecules, vol 22, no. 7, 1192. DOI: 10.3390/molecules22071192
De Deurwaerdère, P, Binda, C, Corne, R, Leone, C, Valeri, A, Valoti, M, Ramsay, RR, Fall, Y & Marco-Contelles, J 2017, 'Comparative analysis of the neurochemical profile and MAO inhibition properties of N-(furan-2-ylmethyl)-N-methylprop-2-yn-1-amine' ACS Chemical Neuroscience, vol 8, no. 5, cn-2016-00377h, pp. 1026-1035. DOI: 10.1021/acschemneuro.6b00377
Deurwaerdère, P, Ramsay, RR & Di Giovanni, G 2017, 'Neurobiology and neuropharmacology of monoaminergic systems' Progress in Neurobiology, vol 151, PRONEU 1480, pp. 1-3. DOI: 10.1016/j.pneurobio.2017.02.001
Hroch, L, Guest, P, Benek, O, Soukup, O, Janockova, J, Dolezal, R, Kuca, K, Aitken, L, Smith, TK, Gunn-Moore, F, Zala, D, Ramsay, RR & Musilek, K 2017, 'Synthesis and evaluation of frentizole-based indolyl thiourea analogues as MAO/ABAD inhibitors for Alzheimer’s disease treatment' Bioorganic & Medicinal Chemistry, vol 25, no. 3, pp. 1143-1152. DOI: 10.1016/j.bmc.2016.12.029
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Dr Rona Ramsay
Biomolecular Sciences Building
University of St Andrews
North Haugh
St Andrews
Fife
KY16 9ST
UK

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