Ms Stefanie Menzies

PhD Student

BMS B305
tel. 01334 463417

Understanding the Chemistry and Biology of a Novel Class of Natural Product-like Inhibitors of Trypanosomatids

Stefanie is currently on a four-year PhD studentship funded by the Leverhulme Trust and St Andrews 600th Anniversary. Jointly supervised by Prof. Terry Smith and Dr. Gordon Florence.

Her research focuses on drug discovery for Neglected tropical diseases.  Trypanosomatid parasites infect millions of people, causing the diseases African Sleeping Sickness, Chagas Disease and Leishmaniasis. These diseases cause high morbidity and inevitable mortality, and current drug treatments are associated with severe adverse effects and are largely ineffective.   Her primary project is to determine how the natural product chamuvarinin and its derivatives are able to kill these parasites.  To answer this, she has tested a library of analogues against the parasites to establish which structural features of the compounds are important for inhibitory activity, allowing more effective and selective compounds to be synthesized.  Stefanie has shown that the protein target of chamuvarinin is mitochondrial and involved in mitochondrial respiration and is continuing investigations into the exact protein target.  Her secondary project is to investigate the function and essentiality of a novel and unique protein in these parasites, which despite being conserved between all three parasites and absent in humans, has not yet been investigated as a potential drug target. She has shown that the protein is essential to these parasites and is located in the mitochondria.  She is currently establishing an enzyme assay to understand its function and to screen for inhibitors for drug discovery.    

Stefanie studied B.Sc (Hons) Medical Sciences at the University of Leeds, with an honours project in the association between paediatric respiratory syncytial virus infection and development of childhood asthma. She then went on to study M.Sc. Biology and Control of Parasites and Disease Vectors at the Liverpool School of Tropical Medicine. Her Masters dissertation was to investigate whether infection with soil-transmitted helminths increased susceptibility to respiratory virus infection, conducted with Prof. Phil Cooper in rural Ecuador as part of the ongoing cohort study FEPIS (Fundacion Ecuatoriana Para Investigacion en la Salud). After graduating Stefanie continued her work with FEPIS as a research assistant, during which time she was responsible for projects investigating; risk factors for soil-transmitted helminth infections in infants, investigations into the aetiology of non-rotavirus diarrhoea in infants in rural Ecuador, and analysing IgE levels to common Ecuadorian allergens and whether this influenced development of asthma.






Menzies, SK, Tulloch, LB, Florence, GJ & Smith, TK 2018, 'The trypanosome alternative oxidase: a potential drug target?' Parasitology, vol 145, no. 2, pp. 175-183. DOI: 10.1017/S0031182016002109
Fraser, AL, Menzies, SK, King, EFB, Tulloch, L, Gould, ER, Zacharova, M, Smith, TK & Florence, GJ 2018, 'Design and synthesis of broad spectrum trypanosomatid selective inhibitors' ACS Infectious Diseases, vol In press. DOI: 10.1021/acsinfecdis.7b00187
Tulloch, LB, Menzies, SK, Coron, RP, Roberts, MD, Florence, GJ & Smith, TK 2018, 'Direct and indirect approaches to identify drug modes of action' IUBMB Life, vol 70, no. 1, pp. 9-22. DOI: 10.1002/iub.1697
Gould, ER, King, EFB, Menzies, SK, Fraser, AL, Tulloch, LB, Zacharova, MK, Smith, TK & Florence, GJ 2017, 'Simplifying nature: towards the design of broad spectrum kinetoplastid inhibitors, inspired by acetogenins' Bioorganic & Medicinal Chemistry, vol 25, no. 22, pp. 6126-6136. DOI: 10.1016/j.bmc.2017.01.021
Menzies, SK, Tulloch, LB, Fraser, AL, Gould, ER, King, EF, Zacharova, MK, Florence, GJ & Smith, TK 2017, 'ANALOGS OF THE NATURAL PRODUCT CHAMUVARININ TARGET THE TRYPANOSOMATID FOF1-ATP SYNTHASE MITOCHONDRIAL COMPLEX V' American Journal of Tropical Medicine and Hygiene, vol 95, no. 5, pp. 164-164.
1  2 
Ms Stefanie Menzies
Biomolecular Sciences Building
University of St Andrews
North Haugh
St Andrews
KY16 9ST

Other pages related to
Ms Menzies: